Rhamnolipids from Pseudomonas aeruginosa Rn19a Modifies the Biofilm Formation over a Borosilicate Surface by Clinical Isolates

Microbial cells are reversibly associated with surfaces in the form of biofilms. Adhesion is mechanism used by microorganisms to bind a surface initially; no biofilm formed without initial adhesion. The aim this work was evaluate efficacy rhamnolipids Pseudomonas aeruginosa Rn19a inhibiting biofilms clinical isolates Escherichia coli I5, E26, Enterococcus faecalis I27 on borosilicate coupons inside Center for Disease Control and Prevention (CDC) reactor. isolate E26 (P. aeruginosa) did not show an adverse effect formation rhamnolipid presence showed normal growth all conditions tested (dynamic static growth). decreased its ability 2.2 log CFU/cm2 addition 3.0 units dynamic conditions. Finally, E. I5 more susceptible influence coupon covered rhamnolipids. E5 reduced capacity at 6.0 Biofilm also observed Confocal Laser Scanning Microscopy. In summary, application may be useful prevent adhesion bacteria surfaces. At minimum, effectively inhibit or diminish biofilm-forming that do belong genus Pseudomonas.